Comparison between in vitro toxicities of tobacco- and menthol-flavored electronic cigarette liquids on human middle ear epithelial cells
Identifieur interne : 000182 ( an2020/Analysis ); précédent : 000181; suivant : 000183Comparison between in vitro toxicities of tobacco- and menthol-flavored electronic cigarette liquids on human middle ear epithelial cells
Auteurs : Yoon Young Go [Corée du Sud] ; Ji Yoen Mun [Corée du Sud] ; Sung-Won Chae [Corée du Sud] ; Jiwon Chang [Corée du Sud] ; Jae-Jun Song [Corée du Sud]Source :
- Scientific Reports [ 2045-2322 ] ; 2020.
Abstract
Since electronic-cigarettes (e-cigarettes) are considered less toxic than conventional tobacco smoking, the use of e-cigarettes has increased, and the market for e-cigarette liquids (e-liquids) is continuously increasing. However, many studies showed that e-cigarettes may cause various harmful effects in lung, oral and heart. In this study, we investigated the effects of e-liquids on otitis media (OM) using human middle ear epithelial cells (HMEECs). Menthol-flavored e-liquid induced significant cell death in HMEECs (IC50: 1.45 ± 0.14%) and tobacco-flavored e-liquid led to increase in inflammatory cytokine levels and higher mucin production. Flavored e-liquids decreased the mRNA levels of genes encoding epithelial sodium channels (ENaCs) in HMEECs. Apoptosis and autophagy reactions were induced by exposure of HMEECs to menthol- and tobacco-flavored e-liquids. Tobacco-flavored e-liquids caused a greater increase in the levels of autophagosome marker, LC3-II, compared to menthol-flavored e-liquids, which was followed by cell death. These results demonstrate that flavored e-liquids cause cytotoxicity via apoptosis, autophagy, inflammatory response, and mucin production in HMEECs. The flavors present in e-liquids might be a risk factor for the development of otitis media.
Url:
DOI: 10.1038/s41598-020-59290-y
PubMed: 32054887
PubMed Central: 7018737
Affiliations:
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id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0840 2678</institution-id><institution-id institution-id-type="GRID">grid.222754.4</institution-id><institution>Department of Otorhinolaryngology-Head and Neck Surgery,</institution><institution>Korea University College of Medicine,</institution></institution-wrap>Seoul, Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Seoul</wicri:regionArea></affiliation></author><author><name sortKey="Mun, Ji Yoen" sort="Mun, Ji Yoen" uniqKey="Mun J" first="Ji Yoen" last="Mun">Ji Yoen Mun</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0840 2678</institution-id><institution-id institution-id-type="GRID">grid.222754.4</institution-id><institution>Department of Otorhinolaryngology-Head and Neck Surgery,</institution><institution>Korea University College of Medicine,</institution></institution-wrap>Seoul, 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Sud</country><wicri:regionArea>Seoul</wicri:regionArea></affiliation></author></analytic><series><title level="j">Scientific Reports</title><idno type="eISSN">2045-2322</idno><imprint><date when="2020">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p id="Par1">Since electronic-cigarettes (e-cigarettes) are considered less toxic than conventional tobacco smoking, the use of e-cigarettes has increased, and the market for e-cigarette liquids (e-liquids) is continuously increasing. However, many studies showed that e-cigarettes may cause various harmful effects in lung, oral and heart. In this study, we investigated the effects of e-liquids on otitis media (OM) using human middle ear epithelial cells (HMEECs). Menthol-flavored e-liquid induced significant cell death in HMEECs (IC<sub>50</sub>: 1.45 ± 0.14%) and tobacco-flavored e-liquid led to increase in inflammatory cytokine levels and higher mucin production. Flavored e-liquids decreased the mRNA levels of genes encoding epithelial sodium channels (ENaCs) in HMEECs. Apoptosis and autophagy reactions were induced by exposure of HMEECs to menthol- and tobacco-flavored e-liquids. Tobacco-flavored e-liquids caused a greater increase in the levels of autophagosome marker, LC3-II, compared to menthol-flavored e-liquids, which was followed by cell death. These results demonstrate that flavored e-liquids cause cytotoxicity via apoptosis, autophagy, inflammatory response, and mucin production in HMEECs. The flavors present in e-liquids might be a risk factor for the development of otitis media.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Teele, Dw" uniqKey="Teele D">DW Teele</name></author><author><name sortKey="Klein, Jo" uniqKey="Klein J">JO Klein</name></author><author><name sortKey="Rosner, B" uniqKey="Rosner B">B Rosner</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Eccles, R" uniqKey="Eccles R">R Eccles</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Im, Gj" uniqKey="Im G">GJ Im</name></author><author><name sortKey="Park, Mk" uniqKey="Park M">MK Park</name></author><author><name sortKey="Song, Jj" uniqKey="Song J">JJ Song</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cho, Jg" uniqKey="Cho J">JG Cho</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Song, Jj" uniqKey="Song J">JJ Song</name></author><author><name sortKey="Lee, Jd" uniqKey="Lee J">JD Lee</name></author><author><name sortKey="Lee, Bd" uniqKey="Lee B">BD Lee</name></author><author><name sortKey="Chae, Sw" uniqKey="Chae S">SW Chae</name></author><author><name sortKey="Park, Mk" uniqKey="Park M">MK Park</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chang, J" uniqKey="Chang J">J Chang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Davis, B" uniqKey="Davis B">B Davis</name></author><author><name sortKey="Dang, M" uniqKey="Dang M">M Dang</name></author><author><name sortKey="Kim, J" uniqKey="Kim J">J Kim</name></author><author><name sortKey="Talbot, P" uniqKey="Talbot P">P Talbot</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tang, Moon Shong" uniqKey="Tang M">Moon-shong Tang</name></author><author><name sortKey="Wu, Xue Ru" uniqKey="Wu X">Xue-Ru Wu</name></author><author><name sortKey="Lee, Hyun Wook" uniqKey="Lee H">Hyun-Wook Lee</name></author><author><name sortKey="Xia, Yong" uniqKey="Xia Y">Yong Xia</name></author><author><name sortKey="Deng, Fang Ming" uniqKey="Deng F">Fang-Ming Deng</name></author><author><name sortKey="Moreira, Andre L" uniqKey="Moreira A">Andre L. 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Yoo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sobolewski, Cyril" uniqKey="Sobolewski C">Cyril Sobolewski</name></author><author><name sortKey="Cerella, Claudia" uniqKey="Cerella C">Claudia Cerella</name></author><author><name sortKey="Dicato, Mario" uniqKey="Dicato M">Mario Dicato</name></author><author><name sortKey="Ghibelli, Lina" uniqKey="Ghibelli L">Lina Ghibelli</name></author><author><name sortKey="Diederich, Marc" uniqKey="Diederich M">Marc Diederich</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chun, Ym" uniqKey="Chun Y">YM Chun</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Offman, Mn" uniqKey="Offman M">MN Offman</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>Corée du Sud</li></country></list><tree><country name="Corée du Sud"><noRegion><name sortKey="Go, Yoon Young" sort="Go, Yoon Young" uniqKey="Go Y" first="Yoon Young" last="Go">Yoon Young Go</name></noRegion><name sortKey="Chae, Sung Won" sort="Chae, Sung Won" uniqKey="Chae S" first="Sung-Won" last="Chae">Sung-Won Chae</name><name sortKey="Chang, Jiwon" sort="Chang, Jiwon" uniqKey="Chang J" first="Jiwon" last="Chang">Jiwon Chang</name><name sortKey="Mun, Ji Yoen" sort="Mun, Ji Yoen" uniqKey="Mun J" first="Ji Yoen" last="Mun">Ji Yoen Mun</name><name sortKey="Song, Jae Jun" sort="Song, Jae Jun" uniqKey="Song J" first="Jae-Jun" last="Song">Jae-Jun Song</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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